Cytek Investor Day Presentation Deck
Spectral flow cytometry
Traditional flow cytometers use single lasers to excite a fluorochrome, and detects
fluorescence in narrow bandwidths using dichroic mirrors, filters and PMTs.
cyan laser
Spectral flow cytometry collects 488 nm
fluorochrome data as complete
spectra using multichannel PMTs or
multiplex APD or SiPM arrays.
Rather than compensation, it uses
spectral unmixing or deconvolution
to separate fluorescent probes
(similar to confocal microscopy).
D
V16 V15 V14 V13 V12 V11
V10
V9
Detectors
V8
V4 V3
DS
DS
10⁰-
SSC
10-
PerCP
Cy5.5
488 10
710/50
PE
680LP
552 LP
575/26
PE
TxRed
ultraviolet 355 nm
610/20
595 LP
635 LP
670/30
505 LP
Spectral flow uses all lasers to excite all fluorochromes (not just the most optimal
one) and collects the full spectra of each fluorochrome from all laser sources. The
data is far more granular and allows better spectral separation that traditional
compensation.
750 LP
PE-Cy5
530/30
√3 VA √5
AF488
Her
780/60
Hempty,
PE-Cy7
Positive Alexa Fluor 488 (Cells)
violet 405 nm
cell number
Channel
M
AF488 fluorescence
(530/30 nm)
blue 488 nm
Alexa Fluor 488
yellow 561 nm red 640 nmView entire presentation