Cytek Investor Day Presentation Deck

011100 01011 001 1010001 10001 1010001 10001 1000111 101010101110001011 001 1010001 10001 1010001 10001 10001 11 10101010110001011 001 1010001-1000 1010001 10001 10001 11 101010101110001011001 1010001 10001 10100 10001 1000111101010 48 Class Cell Type Subtypes Granulocytes Neutrophils CD16 and CD66b or CD15/SSEA1+ Myeloid-Derived Suppressor Cells (MDSCS) Eosinophils CD193 and Siglec-8 and CD16- Basophils FceR10 and CD117- Activated CD63 and CD203c Mast cells FceR10' and CD117+ and Tryptase Activated CD203c CYTEK TRANSCEND THE CONVENTIONAL Cell Signar Plasmacytoid Dendritic Cells (pDCs) Monocytic (M) MDSCS CD15-and CD14' and HLA-DR- Myeloid Cells CD14-and HLA-DR- CD83 Polymorphonuclear (PMN) MDSCs CD15 and CD11b- HLA-DR and CD123* Activated Conventional Dendritic Cells (CDCs) Activated CD83+ CD11c and HLA-DR Cell Signaling Macrophages CDC1s (excel at cross- presentation) XCR1 or CLEC9A CDC2s CD1c or SIRPO Monocytes CD68 and HLA-DR and CD11c M1-like CD86'or CD80° or INOS CM2-like CD163 or CD206 Activated CD69 or CD25+ Leukocytes Central Memory CD45RO" and CD62L or CCR7 CD45+ CD14 and HLA-DR and CD206 and CD86- Th9 PU.1 and T Cells Tfh Bcl-6° and CXCR5 and IL-21- Th22 AHR and IL-22 Cytotoxic Granzyme or Perforin Effector CD45RA" and CD62L-or CCR7- CD3 Cytotoxic T Cells CD8+ NKT Cells Helper T Cells (Th) CD4+ Th1 T-Bet' and IFNY Th2 GATA-3-and Th17 RORyt and IL-17- Treg FoxP3 and CD25+ Naive CD45RA and CD62L or CCR7" Progenitor Exhausted TCF1/TCF7* and PD-1 and TIM-3 CD56 and CD3 Type II NKT Type I NKT (INKT) TCR Va24 V$11 Lymphoid Cells Effector Memory CD45RO and CD62L™ or CCR7- NK Cells Terminally Exhausted Tox/Tox2" and PD-1 and TIGIT- CD56 and CD3 PB Immature/ Regulatory NK Cells CD56 and Cell SignaCD16-and TECHTOI NCR Activated CD69 B Cells PB Cytotoxic NK Cells CD56 and CD16 Cytotoxic Granzyme ar Perforin CD19 Plasma Cells Naive IgD-and CD27- Switched Memory IgD-and CD27 Unswitched Igo and CD27 Key BCMA or CD138 *positive/high expression "negative/low expression Functional State Markers rev. 02/26/21 © 2003-2021 Cell Signaling Technology, Inc. es The James THE OHIO STATE UNIVERSITY COMPREHENSIVE CANCER CENTER

View entire presentation