Cytek Investor Day Presentation Deck
side scatter
hCD3 PE-CF594
The simple 12 color T regulatorv cell panel below (designed not for low spectral
overlap but to test fluorochromes spectrally close to CF-5 and R-PC) show that
these probes can be reasonably combined with spectrally similar fluorochromes.
4.0M-
3.0M-
2.0M-
0
1.0M
CD3+
-104
Lymphocytes.
66.2
forward scatter
0
2.0M
3.0M
hCD19 APC
4.0M
hCD14 PE-Cy5
hCD4 R-PC
-104
0
104
CD45+
hCD45 PerCP
105
CD4+
CD8+
104
-10*
hCD8 CryptoFluor-5
10
hCD25 APC-Cy7
hCCR7 PerCP-Cy5.5
-104
104
hCD127 PE-Cy7
104
hCD127 PE-Cy7
-10°
10°
Above. Resting human PBMCs labeled with the 12 indicated antibodies
and analyzed on a Cytek Biosciences Aurora. Right. Spectral indices
matrix. Complexity index was 11.41.
These results indicate that both CryptoFluor-5 and R-
phycocyanin can be used as fluorochromes in high-
dimensional labeling panels for spectral cytometry (although
conjugation conditions need to be optimized). These PBs can
are not tandem dyes, making them potentially more
spectrally uniform options.
hCD25 APC-Cy7
-104
hCCR7 PerCP-Cy5.5
BUV563
BV605
PerCP
PerCP-Cy5.5
PE
CryptoFluor-5
PE-CF594
R-PC
PE-Cy5
PE-Cy7
APC
0
APC-Cy7
Complexity Index: 11.41
104
BUV563
1
10
BV605
PerCP
CYTO 20
VIRTUAL
INTERACTIVE
21
JUNE 7-10
hCD45RO BUV563
PerCP-Cy5.5
______ _
-10%
105 108
hCD45RA PerCP-Cy5.5
PE
0.03 0.19 0.82 1
0
CryptoFluor-5
0
PE-CF594
104
0.29 0.41 0.29 0.23 0.67 1
R-PC
0.19 0.38 0.39 0.33 0.42 0.83
PE-Cy5
0.16 0.04 0.03 0.42 0.29 0.19 0.07 0.05 0.01 0.02 0
0.16 1 0.23 0.19 0.21 0.41 0.38 0.26 0.18 0.03 0.12 0.02
0.04 0.23 1 0.82 0.07 0.29 0.39 0.56 0.81 0.12 0.38 0.07
PE-Cy7
0.06 0.23 0.33 0.52 0.69 0.24 0.35 0.16
0.42 0.21 0.07 0.06 1 0.67 0.42 0.15 0.11 0.03 0.04 0.01
APC
0.07 0.26 0.56 0.52 0.15 0.53 0.53 1
APC-Cy7
0.83 0.53 0.44 0.08 0.21 0.03
0.05 0.18 0.81 0.69 0.11 0.44 0.52 0.85 1
1 0.53 0.52 0.08 0.19 0.02
0.01 0.03 0.12 0.24 0.03 0.08 0.08 0.14 0.14
0.85 0.14 0.74 0.15
0.14 0.52 0.09
1 0.06 0.3
0.02 0.12 0.38 0.35 0.04 0.21 0.19 0.74 0.52 0.06 1 0.18
Telford, DeLonge, Int Veldt, Kapoor, Hawk
and Morseman (CYTO 2021)
0.02 0.07 0.16 0.01 0.03 0.02 0.15 0.09 0.3 0.18 1View entire presentation