Cytek Investor Day Presentation Deck
Our Previous Work Implicate y8 T cells as Inflammatory Driver in HIV, Aging
Multivariate Computational Analysis of Gamma Delta T Cell
Inhibitory Receptor Signatures Reveals the Divergence of
Healthy and ART-Suppressed HIV+ Aging
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Anna C. Belkina¹2, Alina Starchenko³, Katherine A. Drake4, Elizabeth A. Proctor³,
Douglas A. Lauffenburger, Nina Lin5t and Jennifer E. Snyder-Cappione¹,6*+
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CITRUS algorithm:
CD8+ T cells
TIGIT
16-color immunophenotyping (PBMCs)
OMIP-037 (Belkina et al, 2017)
Ce
NK cells
CD56+
CD16+
TIGIT on y8 T
cells stratified
subjects
CD4+ T cells
into HIV+ vs HIV-
groups with 89%
CV accuracy
yo T cells
TIGIT: druggable
checkpoint
inhibitor
(tiragolumab)
analyte conc. (ng/ml)
800-
600-
% TIGIT+ y8 T cells tracked with
plasma inflammatory markers
400-
200-
0
D-Dimer
R²=1301
25
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A
50 75 100
total
% TIGIT+
16 markers of inflammation in plasma
3.0x106-
Riley M. F. Pihl¹, Alex Olson5,
1.5x10-
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0.0+
0
Fibrinogen
R=.2098
25 50 75 100
total
% TIGIT+
Scores on LV 2 (8.88%)
4
2
O
2
-3
-4
-6
-4
&
● Healthy young
● Healthy older
● HIV young
● HIV older
-95% CL
-2 0 2
Scores on LV 1 (17.66%)
4
6
IR signatures on y8 T cells and plasma
markers stratify 4 groups of subjects in a
PLS-DA multivatriate modelView entire presentation