Bruker Corporation 2023 JP Morgan Healthcare Conference
BREAKOUT OPPORTUNITY 4D PROTEOMICS
Research Highlight #3:
timsTOF enabling higher throughput single-cell proteomics
■
-
timsTOF SCP (Single Cell Proteomics) has a robust workflow and very
high sensitivity, with >3,500 proteins identified on average in single cells
▪ Professor Nikolai Slavov at Northeastern University is pioneering
plexDIA to combine sensitivity of Data Independent Analysis (DIA) with
throughput of labeling to quantify > 1,000 protein groups per cell with
only 5 min nanoLC gradients on timsTOF SCP
2023 Bruker
Prof. Nikolai Slavov
plexDIA: Multiplicative scaling
11-plex DIA
3-plexDIA
LF-DIA
18-plex
DDA
Throughput
# Parallel
peptides
# Parallel
samples
a
MS1 MS2, dia-PASEF
timsTOF SCP
lon mobility
Duty cycle time
m/z range
b
Precursors per cell
4000
3000
2000
1000
0
Melanoma
PDAC
U-937
C
1250
Protein groups per cell
1000
750
500
250
d
30
8
Retention time (min)
0
timsTOF
SCP
timsTOF
0
500
Mean precursors/minute
1000
BRUKER
e
Protein Jaccard index
1.00
0.75
0.50
0.25
0.00
Within a run
Across runs
2023 JP Morgan Healthcare Conference | January 9, 2023
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