Cytek Investor Day Presentation Deck
Spectral flow cytometry
Full spectrum flow cytometry provides many advantages over traditional cytometry
Dramatically improved ability to separate the signals from fluorescent probes
with similar (but non-identical) spectra. This allows us to analyze many more
fluorescent probes (and cell markers) simultaneously. More than 40 marker
analysis is now practical (and has been reported).
Improved quality of signal separation. While spectral cytometry is not strictly
more sensitive than traditional techniques, improved signal separation
improves data quality.
Some interesting advantages derive from these improvements...
Spectral cytometry is more "forgiving" of less-than-optimal fluorochrome
selections.
Many older fluorochromes previously less useful for flow cytometry now have
new potential. The variety of fluorescent probes now usable for cytometry is
greatly increased. Virtually any visible fluorochrome can be used.
Spectral analysis allows improved subtraction of cellular autofluorescence,
improving detection sensitivity, particularly in difficult cell types like myeloid
lineages and tumors.View entire presentation