Cytek Investor Presentation Deck
First 40-color Optimized Multicolor Immunofluorescence Panel
ISAC CYTOMETRY
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OMIP-069: Forty-Color Full Spectrum Flow Cytometry
Panel for Deep Immunophenotyping of Major Cell
Subsets in Human Peripheral Blood
Lily M. Park,' Joanne Lannigan, Maria C. Jaimes"
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PURPOSE AND APPROPRIATE SAMPLE TYPES
This 4-o camery-based panel w developed for in-depth i
OMIP
M
Viimot
of the Sample
en belated, especially in
altri material when
tiple type af
ingannequined from a single sample or inpoint Maximizing the
on that can be used from de sarpel
mon in-depth duracterization of the impatto add the
fple ability. The pred pred hem de CD4 Tel
CDI call, alory Todd, y T celle, NT Lock, 5 celle, NC cells, monac
and details Fit mich gecike all tyge, the past includes refer
by changes of action and direk
well as chemokine coptan Mower, the combination of driple makers in one
tight ked to the very of mis phenotype and their
in
outain di
Ct nane, this panel was desigrand to include only rice carot
the need for Esstion and permocions The pand can be for s
characng the reasa pens in the of infoarte
las
imme amtaring cancer patients
patiens se inmuno chemothes, and
discovery of anique and table bomarkers. Derent from al
from all previa
OMIPS, this past was developed using a full spectrum four contra technology
that had the affectree of lacrescent markens in a single pand. The pand
Buruz paphand bad ma
Key torms
Aussa; haud immunophenotyping full spectra high-dinara
OM
was developed wing sysposurved
(PBMC) foky adsko Table 1 Although we have not reste ganciones
PRMO wa had, it is stated that the palad been the
with furter op 20 BI
c
PRMC
BACKGROUND
The ad ukrad the much
akher post matherapy and
and pathways of va
posso cascar, wat
das que me and wish will
Grecandination of inmate subpag-
der profing of the imate
were informed decisions garding the Haration of li
bons and the development of new thematic appesada. (1-4
Ung the complexity of the human immune impone requins the ability to
periam High-through, in-depth analys there cell and pl
Rew yory has sought to address the need by aouing the character of
single-cell en exssion, through the bending of Dundreds
umap_2
10
13
1 Naive CD41
2 Central Memory CD4
19
3 Early Effector CD4
4 Early Like Effector CD41
5 Early Effector CD4 PD-11
6 CD4 CD57
7 CD4 CD127 CD25" CD45RA
8 CD4 CD127 CD25 CD45RA CD395
9 CD4 CD127 CD25 CD45RA CD39
28
29
umap_1
26
33
34
10 Naive CD8-
11 Central Memory CD8
12 Early Effector CDB
13 Intermediate Effector CD8
14 CD8 CD4SRA CCR7
15 CD8 CD45RA" CCR7
16 CD45RA Terminal Effector CD8+
17 CCR7 CD45RA TCR
18 CCR7 CD45RA TCR
35
Donor 3444
19 CD2 CD8 NKT-like
20 CD2 CD8 NKT-like
21 CD4 CDB
22 CD4 CD6-
23 ILCs
24 Early NK
25 Mature NK
26 CD159 NK
27 Mature NK CD159c
Donor 3458
umap_1
28 IgD CD27 B cells
29 IgD CD27 8 cells
30 igD Memory
31 lgG B cells
32 PDCs
33 CD1C DCs
34 CD1c- CD141 DCs
CD45 CD123 HLA-DR Besophils
Classical Monocytes
Donor 4559
Donor 5036
Intermediate Monocytes
Non-classical Monocytes
Number of
colors/sotopes
50
40
30
10
0
First publication to go beyond 28-color fluorescence flow cytometry, exceeding OMIP
panels published using mass cytometry
40-color panel covering almost the entire cellular composition of the human peripheral
immune system with a single tube
Higher throughput, robust population resolution and lower cost
n = 2
Mass cytometry
Full spectrum flow
cytometry
2010
OLL
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11
5
5
6
2011 2012 2013 2014 2015
5
2016
Publication year
↓
OC
5
10
2017 2018
CYTEK
TRANSCEND THE CONVENTIONAL
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2019 2020
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